Discussing zebrafish genetic methods

This blog was originally set up to help plan a workshop held at the 2007 SCZI. During this workshop, it was clear that while there are some powerful new advances in genetic methods for the zebrafish, there are important questions about some existing methods, and several methods (notably homologous recombination and RNAi) which would be great for the field, but do not yet exist.

We've left this blog up as a forum in which people can discuss some of these methods. Below are several blog entries describing particular methods. Please add questions and comments under the appropriate heading.

--Chi-Bin Chien, Koichi Kawakami, Todd Evans, Hazel Sive

Monday, January 22, 2007

Community resources

Here are some resources that might be useful to develop as a community:

• ORFeome libraries (coding sequences in Gateway entry vectors)
• enhancer libraries (enhancers or enhancer/promoters in Gateway entry vectors)
• Gal4 enhancer trap lines
• tissue-specific Cre lines

We note that distribution of fish lines will be laborious. ZIRC is working on a model where they visit the screening lab and cryopreserve sperm on-site.

It would be very useful to have the community's comments on the utility of ORFeome, enhancerome, or promoterome libraries.
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2 comments:

Anonymous said...

Hazel Sive points out that if RNAi can be made to work, libraries of RNAi sequences targeting all genes would be extremely useful.

January 26, 2007 at 4:52 AM
Lara Hutson said...

Since we use GAL4/UAS quite a bit, I would love to see promoter libraries driving GAL4.

January 26, 2007 at 7:32 AM

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