In flies, the Gal4/UAS system is invaluable for driving tissue-specific expression of transgenes. In mice, the Cre/Lox system and more recently, Cre/Lox combined with Tet on/off or regulatable Cre (e.g. CreER) has played a similar role.
Several labs are conducting Gal4-enhancer trap screens, including Koichi Kawakami's, Herwig Baier's, Marnie Halpern's, and Steve Johnson's. Papers describing pilot screens are in preparation, and most labs seem to be planning to post images of their insertions and make the lines available to the community.
There has recently been published work from several labs, including Tom Look's, on Cre/Lox. Laura Bally-Cuif's lab has had some success with CreERT.
It is not clear how well Cre works--for instance, does recombination recur in every single cell that expresses Cre?
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I would like to see strong evidence that GAL4 activates its target in all cells, not just in a variegated fashion (see Sagasti et al. Curr. Biology 2005).
I also would like to see strong evidence that Cre expressed from an integrated transgene efficiently induces recombination of an integrated transgene in all cells, not just in very few cells.
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