There have been published results showing in vivo electroporation, lipofection, and viral infection in zebrafish embryos; the electroporation methods seem likely to be the most generally applicable. Jon Clarke and Steve Wilson's labs have shown very nice results with single-cell in vivo electroporation.
Global heat-shock of embryos and larvae is becoming very widely used as a method for expressing gene products at particular times (for instance to do late rescue of a mutant).
The Chien lab has also developed a very low-tech, reliable method for local heat-shock induction (using local heating with a modified soldering iron rather than sublethal laser illumination). A manuscript is in preparation; if you email Chi-Bin after about 1 March, we should be able to send you a draft.
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I would be very intersted in hearing about the local heat shock method and any other tips/pitfalls of about using the heat shock system.
Hi there, it would be useful to know who left the comment rather than just "anonymous". Thanks!
I would also be interested in hearing about the local heat shock method
I would also be very interested hearing about this technique
Sorry, I am anonymous!
Good words.
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